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RK13

規格:

貨期:

編號:B162625

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

RK13

商品貨號

B162625

Organism

Oryctolagus cuniculus, rabbit

Tissue

kidney

Product Format

frozen

Morphology

epithelial

Culture Properties

adherent

Biosafety Level

2 [Cells contain Bovine Viral Diarrhea Virus (BVDV)]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

normal

Age

5 weeks

Applications

This cell line is a suitable transfection host.

Storage Conditions

liquid nitrogen vapor phase

Genes Expressed

keratin

Cellular Products

keratin

Virus Susceptibility

Herpes simplex virus
Pseudorabies virus
Vaccinia virus
Rabbitpox virus
Myxoma virus
Simian adenovirus 11
Simian adenovirus 17
Simian adenovirus 3
Simian adenovirus 1
Simian adenovirus 20
Simian adenovirus 18
Simian adenovirus 16
Simian adenovirus 8
Simian adenovirus 19
Simian adenovirus 21
Simian adenovirus 25
Simian adenovirus 22
Simian adenovirus 23
Simian adenovirus 38
Simian adenovirus 37
Simian adenovirus 27
Simian adenovirus 27
Simian adenovirus 39
Simian adenovirus 32
Simian adenovirus 34
Simian adenovirus 31
Simian adenovirus 33
Simian adenovirus 36
Rubella virus , Rubella virus

Comments

The cells are positive for keratin by immunoperoxidase staining. The cells are positive for bovine viral diarrhea virus (BVDV).

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Volumes are given for a 75 cm² flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.

Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended

Medium Renewal: Twice per week

Cryopreservation

Freeze medium: culture medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Atmosphere: air, 95%; carbon dioxide (CO₂), 5%

Temperature: 37°C

Name of Depositor

BC Meyer

Deposited As

Oryctolagus cuniculus

References

Didier ES, et al. Characterization of Encephalitozoon (Septata) intestinailis isolates cultured from nasal mucosa and bronchoalveolar lavage fluids of two AIDS patients. J. Eukaryot. Microbiol. 43: 34-43, 1996. PubMed: 8563708

Beale AJ, et al. Rabbit Cells Susceptible to Rubella Virus. The Lancet 282: 640-641 1963

Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438

McCarthy K, et al. Isolation of Rubella Virus from Cases in Britain. The Lancet 282: 593-598 1963. PubMed: 14050876

Loffler S, et al. CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J. Virol. 71: 42-49, 1997. PubMed: 8985321

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