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當前位置: 首頁 > ATCC代理 > pCK92 [hMCT1] B162642
pCK92 [hMCT1]
pCK92 [hMCT1]
規格:
貨期:
編號:B162642
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pCK92 [hMCT1]
商品貨號
B162642
Designations
pCK92 [hMCT1]
GenBank Number

L31801

Species
Homo sapiens, human
Vector
Construct size (kb): 5.5
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: SalI
Vector end: PstI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: SLC16A1
Gene name: solute carrier family 16 (monocarboxylate transporters),
member 1
Contains complete coding sequence?: Y
Chromosome: 1; Localization: 1 p13.2-p12
Tissue: heart
Type of DNA: cDNA
Insert 5' end: SalI
Insert 3' end: PstI
Insert size (kb): 2.5
Cross references: DNA Seq. Acc.: L31801
Insert lengths(kb): 2.5
Tissue: heart
Gene product: mevalonate transporter(monocarboxylate transporter 1) [SLC16A1]
Insert Size (kb)
2.5
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRV--4.1, 1.4; PstI--5.5; PstI/SalI--2.9, 2.6; XhoI--5.5; BamHI--5.5.
The insert contains the following restriction sites (approximate kb from the 5' end): EcoRV--0.2, 1.6.
Contains the complete coding sequence plus the 3' untranslated region of approximately 1 kb.
References

Garcia CK, et al. cDNA cloning of the human monocarboxylate transporter 1 and chromosomal localization of the SLC16A1 locus to 1p13.2-p12. Genomics 23: 500-503, 1994. PubMed: 7835905

Joseph L Goldstein, personal communication

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