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G355-5
G355-5
規格:
貨期:
編號:B162691
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
G355-5
商品貨號
B162691
Organism
Felis catus, cat
Tissue
brain
Cell Type
astrocyte
Product Format
frozen
Morphology
glial, astrocyte
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
normal
Age
embryo
Applications
This is a cloned derivative of the G355 cell line originally produced and distributed by the Naval Biosciences Laboratory.
The line is useful for generating stocks of various C type retroviruses.
Derivation
This is a cloned derivative of the G355 cell line originally produced and distributed by the Naval Biosciences Laboratory.
Comments
This is a cloned derivative of the G355 cell line originally produced and distributed by the Naval Biosciences Laboratory.
The line is useful for generating stocks of various C type retroviruses.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium:
  • heat-inactivated fetal bovine serum to a final concentration of 10%
    • Subculturing
    Subcultivation Ratio: A subcultivation ratio of 1:5 is recommended
    Medium Renewal: 2 to 3 times per week
    Remove medium, and rinse the monolayer with fresh 0.25% trypsin, 0.03% EDTA solution.
    Remove the trypsin, add fresh trypsin (1 to 2 ml) and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes).
    Add fresh medium, aspirate and dispense into new flasks.
    Culture Conditions
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Name of Depositor
    KJ Dunn
    Deposited As
    Felis catus
    References

    Haapala DK, et al. Isolation from cats of an endogenous type C virus with a novel envelope glycoprotein. J. Virol. 53: 827-833, 1985. PubMed: 2983093

    Bassin RH, et al. Normal DBA/2 mouse cells synthesize a glycoprotein which interferes with MCF virus infection. Virology 123: 139-151, 1982. PubMed: 6959413

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