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當前位置: 首頁 > ATCC代理 > NCI-BL1672 [BL1672] B162722
NCI-BL1672 [BL1672]
NCI-BL1672 [BL1672]
規格:
貨期:
編號:B162722
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
NCI-BL1672 [BL1672]
商品貨號
B162722
Organism
Homo sapiens, human
Tissue
peripheral blood
Cell Type
B lymphoblast; Epstein-Barr virus (EBV) transformed
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
2 [Cells contain Epstein-Barr Virus]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
normal
Age
58 years
Gender
male
Ethnicity
Caucasian
Storage Conditions
liquid nitrogen vapor phase
Comments
A classic small cell lung cancer cell line from this patient is available as ATCC CRL-5886.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 106 viable cells/mL. Maintain cell density between 1 x 106 and 3 x 106 cells/mL.
Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density).
Cryopreservation
Culture medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 11,13
D16S539: 9,11
D5S818: 11,13
D7S820: 10
THO1: 6,10
TPOX: 11,12
vWA: 17
Name of Depositor
AF Gazdar, JD Minna
Deposited As
Homo sapiens
References

NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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