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Human Coxsackievirus B3
Human Coxsackievirus B3
規格:
貨期:
編號:B162778
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Human Coxsackievirus B3
商品貨號
B162778
Classification
Picornaviridae, Enterovirus, Human enterovirus B
Deposited As
Coxsackievirus B3
Agent
Human Coxsackievirus B3
Strain
Nancy
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format
frozen 1 mL per vial
Storage Conditions
-70°C or colder
Comments
Cryopreserved in 10% FBS.
Effect on Host
CPE, refractile rounding and sloughing
Recommended Host
LLC-MK2 (ATCC® CCL-7.1™)
Growth Conditions
Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 1-4 days
Name of Depositor
JL Melnick
Source
Stool from febrile patient with minor illness, Connecticut, 1949
References

Melnick JL, Ledinko N. Immunological reactions of the Coxsackie viruses. I. The neutralization test; technic and application. J. Exp. Med. 92: 463-482, 1950. PubMed: 14778925

Cross References

Nucleotide (GenBank) : AJ295194 Human coxsackievirus B3 genomic RNA for partial polyprotein gene, isolate VR-30, ATCC.

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