Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.
Incubation: 9-16 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Sweet BH and Hilleman MR. The vacuolating virus, S.V.40. Proc Soc Exp Biol Med 105:420-427, 1960. PubMed: 13774265
Lednicky J and Folk WR. Two synthetic Sp1-binding sites functionally substitute for the 21-base-pair repeat region to activate simian virus 40 growth in CV-1 cells. J Virol 66(11): 6379-6390, 1992. PubMed: 1328672
Stewart AR, et al. Identification of a variable region at the carboxy terminus of SV40 large T-antigen. Virology 221(2):355-361, 1996. PubMed: 8661447
Forsman ZH, et al. Phylogenetic analysis of polyomavirus simian virus 40 from monkeys and humans reveals genetic variation. J Virol 78(17): 9306-9316, 2004. PubMed: 15308725
Swain JL, et al. Effects of route of inoculation and viral genetic variation on antibody responses to polyomavirus SV40 in Syrian golden hamsters. Comp Med 62(5):400-408, 2012. PubMed: 23114044
Zhang S, et al. Viral microRNA effects on pathogenesis of polyomavirus SV40 infections in Syrian golden hamsters. PLoS Pathog 10(2):e1003912, 2004. PubMed: 24516384
