Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
ATCC® VR-1875™ was derived from ATCC® VR-515™ following treatment with diethyl ether to remove mycoplasma contamination.
Recognized Reference Strain: Collaborative Report on Rhinoviruses, Nature (Lond.) 213: 761, 1967.
Temperature: 33°C
Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g., 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g., 1.0). Adsorb 1.5-2.0 hours at 33°C in a humidified 5% CO2 atmosphere on a rocking platform. End adsorption by adding virus growth medium.
Incubation: 3-5 days at 33°C in a humidified 5% CO2 atmosphere on a rocking platform, until CPE is progressed through 80% of the monolayer.
Kapikian A, et al. Characterization of two newly recognized rhinovirus serotypes of human origin. Proc. Soc. Exp. Biol. Med. 122: 1155-1162, 1966. PubMed: 4288216
Kapikian AZ et al. Rhinoviruses: a numbering system. Nature 213: 761-762, 1967. PubMed: 4291698
