Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Temperature: 37°C
Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g., 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g., 0.01). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.
Incubation: 1-2 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Mavian C, et al. Emergence of recombinant Mayaro virus strain from the Amazon basin. Sci Rep 7: 8718, 2017.
Long KC, et al. Experimental transmission of Mayaro virus by Aedes aegypti. Am J Trop Med Hyg 85(4): 750-757, 2011. PubMed: 21976583
Raimunda SS, et al. Mayaro Fever Virus, Brazilian Amazon. Emerg Infect Dis 15(11): 1830-1832, 2009.
Lavergne A, et al. Mayaro virus: complete nucleotide sequence and phylogenetic relationships with other alphaviruses. Virus Res 117(2): 283-290, 2006. PubMed: 16343676
