Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Temperature: 28°C
Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g., 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g., 0.01). Adsorb 1-2 hours at 28°C in a humidified, air atmosphere incubator. End adsorption by adding virus growth medium.
Incubation: 6-8 days at 28°C in a humidified, air atmosphere incubator, until CPE is progressed through 80% of the monolayer.
Suitor EC, Weiss E. Isolation of a rickettsialike microorganism (Wolbachia persica n. sp.) from Argas persicus (Oken). J. Infect. Dis. 108: 95-106, 1961.
Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.
Larson MA, et al. Reclassification of Wolbachia persica as Francisella persica comb. nov. and emended description of the family Francisellaceae. Int. J. Syst. Evol. Microbiol. 66(3): 1200-1205, 2016. PubMed: 26747442
Nucleotide (GenBank) : M21292 Wolbachia persica 16S ribosomal RNA.
