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Human Coxsackievirus B4
Human Coxsackievirus B4
規(guī)格:
貨期:
編號:B162872
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
Human Coxsackievirus B4
商品貨號
B162872
Classification
Picornaviridae, Enterovirus, Enterovirus B
Deposited As
Coxsackievirus B 4
Agent
Human Coxsackievirus B4
Strain
J.V.B. (Benschoten)
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
Stool from 10-year-old child, New York, 1951
Product Format
frozen 1.0 mL
Storage Conditions
-70°C or colder
Images
ATCC VR-184 images
Effect on Host
CPE, cell rounding and sloughing
Recommended Host
LLC-MK2 Derivative (ATCC® CCL-7.1™)
Growth Conditions
Temperature: 37°C
Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-7 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Name of Depositor
H Plager
Source
Stool from 10-year-old child, New York, 1951
Year of Origin
1951
References

Dalldorf G. The Coxsackie viruses. Bull NY Acad.. Med 26: 329-335, 1950. PubMed: 15411582

Sickles GM, et al. Isolation and type determination of Coxackie virus, group B, in tissue culture. Proc. Soc. Exp. Biol. Med. 88: 22-24, 1955. PubMed: 14357332

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