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當(dāng)前位置: 首頁 > ATCC代理 > Human gammaherpesvirus 4 (HHV-4) B162918
Human gammaherpesvirus 4 (HHV-4)
Human gammaherpesvirus 4 (HHV-4)
規(guī)格:
貨期:
編號:B162918
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
Human gammaherpesvirus 4 (HHV-4)
商品貨號
B162918
Classification
Herpesviridae, Lymphocryptovirus
Deposited As
Burkitt's lymphoma
Agent
Human gammaherpesvirus 4 (HHV-4)
Strain
HR1 (clone of P3)
Common Name
Epstein-Barr virus
Applications
The virus is not detected by PCR primers targeting EBNA-2, EBNA-3 regions or by immunofluorescence for anti-VCA and anti-EBNA. Virus was detected by monoclonal antibody against EBNA-1 from Chemicon cat# MAB8173.
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format
frozen
Storage Conditions
vapor phase of liquid nitrogen
Comments
The cells are persistently infected with Human herpesvirus 4 (formerly Epstein-Barr virus, EBV). The HR-1 strain of Epstein-Barr virus (EBV) has a deletion in the EBNA2 gene. The virus is not detected by PCR primers targeting EBNA-2, EBNA-3 regions or by immunofluorescence for anti-VCA and anti-EBNA. Virus was detected by monoclonal antibody against EBNA-1 from Chemicon cat# MAB8173.
Effect on Host
Yes, in vitro effects: Polykaryocyte formation, cell swelling
Polykaryocyte formation, cell swelling
Recommended Host

Host of Choice: Human lymphoblastoid cells

Host Range: Human lymphoblastoid cells (NC37, F265)

Growth Conditions
Temperature: 37.0°C
Max Temperature: 37.0°C
Min Temperature: 35.0°C
Duration: 5-12 days
Protocol: Thaw rapidly, resuspend the ampule contents in 10 ml of cold medium, centrifuge at 800 X g for 5 min, remove the supernatant and resuspend the cells in fetal bovine serum-suplemented medium in a small culture flask filled to a depth of 2 to 3 mm.
Cryopreservation
Storage temperature: liquid nitrogen vapor phase
Subcultivation
Protocol: Thaw rapidly, resuspend the ampule contents in 10 ml of cold medium, centrifuge at 800 X g for 5 min, remove the supernatant and resuspend the cells in fetal bovine serum-suplemented medium in a small culture flask filled to a depth of 2 to 3 mm.
Name of Depositor
FT Buscheck
Special Collection
ATCC
References

Hinuma Y, Grace JT Jr.. Cloning of immunoglobulin-producing human leukemic and lymphoma cells in long-term cultures. Proc. Soc. Exp. Biol. Med. 124: 107-111, 1967. PubMed: 4163469

This virus is continuously produced by the cell line in which it is shipped. No additional host is required, however, if desired the virus may be used to infect other cells which are listed as alternate hosts.

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