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Dengue virus type 2
Dengue virus type 2
規格:
貨期:
編號:B162928
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Dengue virus type 2
商品貨號
B162928
Classification
Flaviviridae, Flavivirus, Dengue virus
Agent
Dengue virus type 2
Strain
New Guinea C
Applications
Vector borne research
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format
frozen 1 mL per vial
Storage Conditions
-70°C or colder
Comments
Derived by adaptation from in vivo product ATCC® VR-1255™ to LLC-MK2 Derivative cells (ATCC® CCL-7.1™). ATCC® VR-1255™ and ATCC® VR-1584™ have not been compared for sequence or infectivity in in vivo and tissue culture.
Effect on Host
CPE, cell aggregation, sloughing, death
Recommended Host
LLC-MK2 Derivative (ATCC® CCL-7.1™)
Growth Conditions
Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 70-80% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 6-8 days
Name of Depositor
CL Wisseman, University of Maryland School of Medicine; Yale Arbovirus Research Unit; National Institute of Allergy and Infectious Diseases; ATCC?
Source
Serum of febrile patient, New Guinea, 1944
References

Sabin AB. Research on dengue during World War II. Am. J. Trop. Med. Hyg. 1: 30-50, 1952. PubMed: 14903434

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