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Human echovirus 9
Human echovirus 9
規格:
貨期:
編號:B162945
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Human echovirus 9
商品貨號
B162945
Classification
Picornaviridae, Enterovirus, Enterovirus B
Deposited As
Echovirus 9
Agent
Human echovirus 9
Strain
Hill
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
Rectal swab from healthy child, Ohio
Product Format
frozen
Storage Conditions
-70°C or colder (or colder)
Comments
This strain is not pathogenic for newborn mice. Related antigenically to Coxsackievirus Type A23. Wigand, R. et al., Arch. Gesamte Virusforsch. 11: 708, 1962.
Effect on Host
Yes, in vitro effects: Cytopathic effects (refractile rounding and sloughing)
CPE, refractile rounding and sloughing
Growth Medium
Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 98%; fetal bovine serum, 2%
Recommended Host

Recommended Host:? LLC-MK2 (ATCC CCL-7)

Growth Conditions
Atmosphere: 5% CO2 in air recommended
Temperature: 37.0°C
Duration: 2-5 days; For best results cells should be 24 to 48 hours old and 80-90% confluent (not 100% confluent) for inoculation.
Cryopreservation
Storage temperature: -70°C
Name of Depositor
HA Wenner
Source
Rectal swab from healthy child, Ohio
References

Ramos-Alvarez M, Sabin AB. Characteristics of poliomyelitis and other enteric viruses recovered in tissue culture from healthy American children. Proc. Soc. Exp. Biol. Med. 87: 655-661, 1954. PubMed: 13237336

Ramos-Alvarez M, Sabin MB. Intestinal viral flora of healthy children demonstrable by monkey kidney tissue culture. Am. J. Public Health 46: 295-299, 1956. PubMed: 13292578

Wigand R, Sabin AB. Antigenic purity and plaque properties of the prototype strains of ECHO virus types 7 to 11, and 17 and 18. Arch. Gesamte Virusforsch. 11: 708-717, 1962. PubMed: 14040255

or colder

or colder

Cross References

Nucleotide (GenBank) : U11710 Echovirus 9 Hill polyprotein gene, 5' UTR.

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