Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 40-60% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 15-20 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-7 daysKilham L, Olivier LJ. A latent virus of rats isolated in tissue culture. Virology 7: 428-437, 1959. PubMed: 13669314
