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Human rhinovirus 16
Human rhinovirus 16
規格:
貨期:
編號:B162950
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Human rhinovirus 16
商品貨號
B162950
Classification
Picornaviridae, Enterovirus
Deposited As
Rhinovirus 16
Agent
Human rhinovirus 16
Strain
11757
Common Name
Rhinovirus 16, HRV-16
Applications
Respiratory research
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format
frozen 1 mL per vial
Storage Conditions
-70°C or colder
Images
VR-283
Comments
The pH of the medium should be maintained in a range of 7.0-7.3. Rocking of cultures during adsorption may enhance viral replication and CPE. Deposited as Rhinovirus HRV-16. HA is not demonstrated. Not inactivated by ether.
Effect on Host
CPE, cell rounding and sloughing
Recommended Host
H1-HeLa (ATCC® CRL-1958™)
Growth Conditions
Temperature: 33°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume (e.g. 1 mL per 25 cm2) of virus diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 33°C in a humidified 5% CO2 atmosphere while continuously rocking. End adsorption by adding virus growth medium.
Incubation: 1-7 days in a humidified 5% CO2 atmosphere until 90% of the culture shows CPE.
Name of Depositor
RM Conant, VV Hamparian, The Ohio State University
Special Collection
ATCC
Source
Throat swab from healthy 2-year-old female, Washington, DC, 1960
References

Hamparian VV, Conant RM. Rhinoviruses: a numbering system. Nature 213: 761-763, 1967. PubMed: 4291698

Johnson KM, Rosen L. Characteristics of five newly recognized Enteroviruses recovered from the human oropharynx. Am. J. Epidemiol. 77(1): 15-25, 1963.

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