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Nuclear polyhedrosis virus, Autographa californica
Nuclear polyhedrosis virus, Autographa californica
規格:
貨期:
編號:B163011
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Nuclear polyhedrosis virus, Autographa californica
商品貨號
B163011
Classification
Baculovirus
Deposited As
Nuclear Polyhedrosis Virus, Lymantria dispar (plaque purified)
Agent
Nuclear polyhedrosis virus, Autographa californica
Strain
LdMNPV-Ab-a624
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
derived from wild type virus isolated from a virus-killed population of Lymantria dispar (gypsy moth), Abington, MA
Product Format
freeze-dried
Storage Conditions
-20°C or colder
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Comments
To propagate, rehydrate in water (or thaw), and feed to gypsy moth larvae (and collect hemolymph), or dissolve in 0.1 M Na2CO3, 0.2 M NaCl (pH 10.8) and inoculate gypsy moth cell culture (and collect cell lysate and supernate).
Effect on Host
Yes, in vivo effects: Kills 4th-5th instar Lymantria dispar larvae, 5-7 days
Yes, in vitro effects: nuclear swelling and inclusion bodies in cell culture, 3-4 days
Recommended Host
gypsy moth larvae
gypsy moth larvae and closely related lymantrids; IPLB LDFB, IPLB 652Y, and IPLB EIT cell cultures
Growth Conditions
Temperature: 28°C
Incubation: 7 days 7 days
Name of Depositor
M Shapiro
U.S. Patent
U.S. Patent Number
Source
derived from wild type virus isolated from a virus-killed population of Lymantria dispar (gypsy moth), Abington, MA
References

Shapiro M, et al. More virulent biotype isolated from wild-type virus. US Patent 5,132,220 dated Jul 21 1992

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