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Human herpesvirus 5
Human herpesvirus 5
規(guī)格:
貨期:
編號(hào):B163020
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng)
Human herpesvirus 5
商品貨號(hào)
B163020
Classification
Herpesviridae, Cytomegalovirus
Deposited As
Cytomegalovirus
Agent
Human herpesvirus 5
Strain
Towne
Common Name
Human cytomegalovirus
Applications
Quality control
Sexually Transmitted Disease Research
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format
frozen 1 mL per vial
Shipping Information
Infected viable cells + 7% DMSO
Storage Conditions
Liquid nitrogen vapor phase
Comments
Add contents of thawed ampule to a 80-85% monolayer of HDF cells. If foci of CPE fail to spread by day 11 PI, split infected cells 1:2 and observe for CPE as monolayer reforms.
Attenuated for man, broad antigenicity. Stable for 6 months at -60°C in form of glycerinated cells. Virus primarily cell associated. A long (10-11 day) incubation is required for initial passage of frozen stocks.
Effect on Host
CPE, cells round, become refractile, eventually slough off the flask
Recommended Host
MRC-5 (ATCC® CCL-171™)
Growth Conditions
Temperature: 37°C
Recommendations For Infection: Trypsinize a 2-4 day old cell culture and seed at a density of 105 cells per cm2 (2.5X106 cells/T25 flask; 7.5X 105 cells per T75 flask) using the virus growth medium (VGM). To the freshly trypsinized cells inoculate using a MOI of 0.1. Immediately add additional VGM depending upon the size of the flask used (recommended medium volume for T25 is 5 to 7 mL and for T75 15 to 22.5 mL). Incubate for 24 hours at 37°C in a 5% CO2 atmosphere. Aspirate media and add fresh virus/cell growth medium to remove any traces of DMSO. Continue incubation.
Incubation: 2-7 days in a 5% CO2 air atmosphere in a stationary position until CPE is seen
Name of Depositor
SA Plotkin
Special Collection
NCRR Contract
Source
Urine, man with congenital cytomegalic inclusion disease
References

Plotkin SA, et al. Candidate cytomegalovirus strain for human vaccination. Infect. Immun. 12: 521-527, 1975. PubMed: 170203

Quality Control of Microbiological Transport Systems; Approved Standard. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M40-A.

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