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Equid herpesvirus 1
Equid herpesvirus 1
規(guī)格:
貨期:
編號:B163297
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
Equid herpesvirus 1
商品貨號
B163297
Classification
Herpesviridae, Varicellovirus
Deposited As
Equine herpesvirus type 1
Agent
Equid herpesvirus 1
Strain
438/77
Common Name
Equine abortion virus
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
Aborted equine fetus
Product Format
frozen
Storage Conditions
-70°C or colder
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Images
VR-2229 images
Effect on Host
CPE, cell rounding and sloughing
Recommended Host
E. Derm (NBL-6) (ATCC® CCL-57™)
Growth Conditions
Temperature: 37°C
Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2), optimized for propagation (e.g. MOI 0.01-0.1 recommended). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 1-2 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Name of Depositor
MJ Studdert
U.S. Patent
U.S. Patent Number
Source
Aborted equine fetus
References

Studdert MJ, et al. Differentiation of respiratory and abortigenic isolates of equine herpesvirus 1 by restriction endonucleases. Science 214: 562-564, 1981. PubMed: 6270790

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