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Human parainfluenza virus 1

規格:

價格:詢價

編號:B163393

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

Human parainfluenza virus 1

商品貨號

B163393

Classification

Paramyxoviridae, Respirovirus

Deposited As

Parainfluenza 1

培養基

Agent

Human parainfluenza virus 1

Strain

C35

Applications

Respiratory research

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format

frozen 1 mL per vial

Storage Conditions

-70°C or colder

Comments

Addition of 1 μg/mL trypsin (1:250 Gibco trypsin catalog #27250018) to culture medium is required for optimal growth. Agglutinates both chicken and guinea pig RBC (0.5%) but the latter to a higher titer than the former. Based on electron microscopic evidence, this product may also contain an as yet unidentified polyoma virus.

Virus presence can be detected with Millipore #5019 Parainfluenza 1 Antibody FITC Reagent

Effect on Host

Slight CPE, rounding and sloughing

Recommended Host

LLC-MK₂ cells (ATCC^® CCL-7.1™)

Growth Conditions

Temperature: 37°C

Recommendations For Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm²). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO₂ atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.

Incubation: 5-7 days continuously rocking at 37°C in a humidified 5% CO₂ atmosphere.

Name of Depositor

R Huebner, RM Chanock, JC Hierholzer, A Chappell

Source

Throat swab, 3-year-old boy with acute laryngitis, Washington, DC, 1957

References

Andrewes CH, et al. Para-influenza viruses 1, 2, and 3: Suggested names for recently described myxoviruses. Virology 8: 129-130, 1959. PubMed: 13669331

Chanock RM. . N. Engl. J. Med. 258: 207-213, 1958.

Cross References

Nucleotide (GenBank) : JQ901971.1 Human parainfluenza virus 1 strain ATCC VR-94, partial genome

B244801：Human adenovirus 21

B244635：Human echovirus 31

B244601：Human herpesvirus 5

B244599：DNA from Human herpesvirus 1, strain HF

B244392：Human echovirus 21

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