黄色小说视频-日本少妇高潮抽搐-国内自拍av-天堂中文资源在线-蜜桃视频在线入口www-91久久综合-亚洲视频在线一区-日日夜夜拍-国产精品资源在线观看-欧美激情国产精品免费-人妻少妇久久中文字幕-成人免费无码大片a毛片-香蕉污视频在线观看-日本电影成人-国内精品久久99人妻无码

歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > 10-1.D.2 B163532
10-1.D.2
10-1.D.2
規格:
價格:詢價
編號:B163532
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
10-1.D.2
商品貨號
B163532
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody induces blastogenesis in B cells, and this response is limited to the Lyb 5+ class of B cells.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against Lyb 2.1 mouse B cell (B lymphocyte) antigen
Cellular Products
immunoglobulin; monoclonal antibody; against Lyb 2.1 mouse B cell (B lymphocyte) antigen
Comments
Animals were immunized with spleen cells from DBA/2 mice.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Lyb-2.1 antigen is a mouse B cell differentiation antigen (45000 dalton glycoprotein).
The antibody induces blastogenesis in B cells, and this response is limited to the Lyb 5+ class of B cells.
B cells activated in this way mature into immunoglobulin secreting cells in the presence of T cell replacing factors (medium from Con A treated T cells).
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
    • Subculturing
    Medium Renewal: Every 2 to 3 days
    Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml ay maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
    Isotype
    IgG2b
    Name of Depositor
    DE Mosier
    Deposited As
    mouse (B cell); mouse (myeloma)
    References

    Subbarao B, Mosier DE. Induction of B lymphocyte proliferation by monoclonal anti-Lyb 2 antibody. J. Immunol. 130: 2033-2037, 1983. PubMed: 6601145

    • 王經理
      手機號：17280875617
      QQ：1438578920
    • 胡經理
      手機號：13345964880
      QQ：2438244627
    • 周經理
      手機號：17757487661
      QQ：1296385441
    • 于經理
      手機號：18067160830
      QQ：2088210172
    • 沈經理
      手機號：19548299266
      QQ：2662369050
    • 李經理
      手機號：13626845108
      QQ：972239479
    • 曹經理
      手機號：18662422060
      QQ：3671869992