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當(dāng)前位置: 首頁 > ATCC代理 > 11B11 [BSF-1] B163545
11B11 [BSF-1]
11B11 [BSF-1]
規(guī)格:
價(jià)格:詢價(jià)
編號:B163545
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
11B11 [BSF-1]
商品貨號
B163545
Organism
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody does not cross-react with other lymphokines, and can be used to purify BSF-1 from complex mixtures.

Storage Conditions
liquid nitrogen vapor phase
Derivation
Animals were immunized with purified BSF-1 emulsified in complete Freund's adjuvant.
Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against murine B cell stimulatory factor 1 (BSF-1, interleukin-4, interleukin 4, IL-4, B cell growth factor 1, BCGF-1)
Cellular Products
immunoglobulin; monoclonal antibody; against murine B cell stimulatory factor 1 (BSF-1, interleukin-4, interleukin 4, IL-4, B cell growth factor 1, BCGF-1)
Comments
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
RPMI 1640 medium with L-glutamine, 90%; heat-inactivated fetal bovine serum, 10%
Subculturing
Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 10viable cells/mL. Maintain cultures at a cell concentration between 2 x 105 and 1 x 10cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
70% Culture Media + 20% FBS + 10% DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Isotype
IgG1
Name of Depositor
WE Paul
Deposited As
rat (B cell); mouse (myeloma)
References

Ohara J, Paul WE. Production of a monoclonal antibody to and molecular characterization of B-cell stimulating factor-1. Nature 315: 333-336, 1985. PubMed: 2582266

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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