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12/49
12/49
規(guī)格:
價(jià)格:詢(xún)價(jià)
編號(hào):B163551
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng)
12/49
商品貨號(hào)
B163551
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain
BALB/c (B cell); BALB/c (myeloma)
Applications
The antibody cross-reacts with human H-Y.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.
Clinical Data
Female BALB/c mice were immunized with lymphocytes from male BALB/c mice.
Genes Expressed
immunoglobulin; monoclonal antibody; against H-Y antigen
Cellular Products
immunoglobulin; monoclonal antibody; against H-Y antigen
Tumorigenic
Yes
Effects
Yes, forms ascites in BALB/c mice
Comments
Female BALB/c mice were immunized with lymphocytes from male BALB/c mice.
Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.
The antibody cross-reacts with human H-Y.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype
IgM; kappa light chain
Name of Depositor
Sloan-Kettering Institute for Cancer Research
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Koo GC, et al. Process for sex determination in man by use of monoclonal antibodies to the H-Y antigen. US Patent 4,680,258 dated Jul 14 1987

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