黄色小说视频-日本少妇高潮抽搐-国内自拍av-天堂中文资源在线-蜜桃视频在线入口www-91久久综合-亚洲视频在线一区-日日夜夜拍-国产精品资源在线观看-欧美激情国产精品免费-人妻少妇久久中文字幕-成人免费无码大片a毛片-香蕉污视频在线观看-日本电影成人-国内精品久久99人妻无码

歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > 142-24E5 [1/24E05, 142-24E05, SCRF 35.1] B163570
142-24E5 [1/24E05, 142-24E05, SCRF 35.1]
142-24E5 [1/24E05, 142-24E05, SCRF 35.1]
規格:
價格:詢價
編號:B163570
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
142-24E5 [1/24E05, 142-24E05, SCRF 35.1]
商品貨號
B163570
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension but many cells attach
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Animals were immunized with a synthetic polypeptide with the sequence YREQIKRVKDSDDVPMVLVGNKC corresponding to peptides 96 to 118 of the predicted p21 sequence of the ras (Ha-ras) oncogene, formerly v-ras (H).
The antibody reacts in indirect fluorescence assays of frozen sections and peroxidase stain paraffin embedded cell lines or tissues.
Images
Derivation
Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against a synthetic H/N-ras oncogene peptide
Cellular Products
immunoglobulin; monoclonal antibody; against a synthetic H/N-ras oncogene peptide
Comments
Animals were immunized with a synthetic polypeptide with the sequence YREQIKRVKDSDDVPMVLVGNKC corresponding to peptides 96 to 118 of the predicted p21 sequence of the ras (Ha-ras) oncogene, formerly v-ras (H).
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody recognizes a 23,000 dalton protein in all mammalian cell lines tested, a 38,000 dalton in yeast cells, and proteins of 100,000, 55,000 and 23,000 daltons in the urine of a variety of individuals.
The antibody reacts in indirect fluorescence assays of frozen sections and peroxidase stain paraffin embedded cell lines or tissues.
A culture submitted to the ATCC in February of 2001 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline.
The cells were assayed for mycoplasma, by the Hoechst stain, PCR and the standard culture test, after a six-week period following treatment. All tests were negative.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Maintain cell density between 5 X 10 exp4 and 1 to 2 X 10 exp6 viable cells/ml.
Cryopreservation
Culture medium with 45% fetal bovine serum, 90%; DMSO, 10%
Culture Conditions
Temperature: 37.0°C
Isotype
IgG1; kappa light chain
Name of Depositor
National Cancer Institute
Deposited As
mouse (B cell); mouse (myeloma)
References

Niman HL, Lerner RA. Polypeptide-induced monoclonal receptors to protein ligands. US Patent 5,015,571 dated May 14 1991

Niman HL, Lerner RA. Polypeptide-induced monoclonal receptors to protein ligands. US Patent 5,030,565 dated Jul 9 1991

Chesa PG, et al. Expression of p21ras in normal and malignant human tissues: lack of association with proliferation and malignancy. Proc. Natl. Acad. Sci. USA 84: 3234-3248, 1984. PubMed: 3554234

Habib NA, Wood CB. The expression of ras oncogene in normal and pathological liver disease. Int. Surg. 71: 182-183, 1986. PubMed: 3771121

Bokoch GM, Parkos CA. Identification of novel GTP-binding proteins in the human neutrophil. FEBS Lett. 227: 66-70, 1988. PubMed: 3123273

Luo JC, et al. Expression p21 ras-related protein in the plasma and tissue of patients with adenomas and carcinomas of the colon. Biomarkers 1: 29-33, 1996.

  • 王經理
    手機號：17280875617
    QQ：1438578920
  • 胡經理
    手機號：13345964880
    QQ：2438244627
  • 周經理
    手機號：17757487661
    QQ：1296385441
  • 于經理
    手機號：18067160830
    QQ：2088210172
  • 沈經理
    手機號：19548299266
    QQ：2662369050
  • 李經理
    手機號：13626845108
    QQ：972239479
  • 曹經理
    手機號：18662422060
    QQ：3671869992