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15-79-2
15-79-2
規格:
價格:詢價
編號:B163583
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
15-79-2
商品貨號
B163583
Organism
Mus musculus (pre-B cell); Mus musculus (myeloma), mouse (pre-B cell); mouse (myeloma)
Tissue
liver
培養基
Cell Type
hybridoma: pre-B lymphoblast
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age
fetus
Applications
The cells produce, but do not secrete mu chain.
No light chain is produced.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Fetal liver cells (14 days gestation) were fused with P3X63Ag8.653 myeloma cells.
No light chain is produced.
Genes Expressed
immunoglobulin; mu heavy chain (not secreted)
Cellular Products
immunoglobulin; mu heavy chain (not secreted)
Comments
Fetal liver cells (14 days gestation) were fused with P3X63Ag8.653 myeloma cells.
The cells produce, but do not secrete mu chain.
No light chain is produced.
The kappa light chain genes have not been functionally re-arranged.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
Culture Medium: RPMI medium 1640, 80%; fetal bovine serum, 20%.
Subculturing
Medium Renewal: Every 1 to 2 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Name of Depositor
JF Kearney
Deposited As
mouse (pre-B cell); mouse (myeloma)
References

Burrows P, et al. Evidence that murine pre-B cells synthesize mu heavy chains but no light chains. Nature 280: 838-841, 1979. PubMed: 112480

Perry RP, et al. Organization and expression of immunoglobulin genes in fetal liver hybridomas. Proc. Natl. Acad. Sci. USA 78: 247-251, 1981. PubMed: 6787586

Maki R, et al. Immunoglobulin gene rearrangement in immature B cells. Science 209: 1366-1369, 1980. PubMed: 6774416

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