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158.2 [MA158.2]
158.2 [MA158.2]
規格:
價格:詢價
編號:B163584
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
158.2 [MA158.2]
商品貨號
B163584
Organism
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts weakly with resident mouse peritoneal macrophages and strongly with mouse macrophages that have been activated by treatment with Macrophage Activating Factor (MAF) or thioglycollate.
The antigen is not related to Mac-1, Mac-2 or Mac-3 and does not appear to be strain or sex specific.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against activated mouse macrophages
Cellular Products
immunoglobulin; monoclonal antibody; against activated mouse macrophages
Tumorigenic
Yes
Effects
Yes, forms ascites in nude mice
Comments
Animals were immunized with activated mouse macrophages.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts weakly with resident mouse peritoneal macrophages and strongly with mouse macrophages that have been activated by treatment with Macrophage Activating Factor (MAF) or thioglycollate.
It does not react with rat macrophages nor with a panel of mouse macrophage, monocyte or lymphoid cell lines.
The antigen is not related to Mac-1, Mac-2 or Mac-3 and does not appear to be strain or sex specific.
The antigen is not trypsin sensitive.
Complete Growth Medium
The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water and supplemented with 1.5 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium: heat-inactivated bovine calf serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG2a
Name of Depositor
SmithKline Beckman Corporation
Deposited As
rat (B cell); mouse (myeloma)
U.S. Patent Number
References

Koestler TP. Monoclonal antibody to activated macrophages. US Patent 4,701,408 dated Oct 20 1987

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