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15C6
15C6
規格:
貨期:
編號:B163585
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
15C6
商品貨號
B163585
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Spleen cells were fused with HL-1 Friendly myeloma cells (a derivative of P3X63Ag8.653 that is deficient in both hypoxanthine phosphoribosyl transferase [HPRT] and adenine phosphoribosyl transferase [APRT]).
The antibody can be used for ELISA, immunoprecipitation and Western blot applications.
Storage Conditions
liquid nitrogen vapor phase
Derivation
Spleen cells were fused with HL-1 Friendly myeloma cells (a derivative of P3X63Ag8.653 that is deficient in both hypoxanthine phosphoribosyl transferase [HPRT] and adenine phosphoribosyl transferase [APRT]).
Genes Expressed
immunoglobulin; monoclonal antibody; against the murine cytomegalovirus (MCMV) m144 heavy chain
Cellular Products
immunoglobulin; monoclonal antibody; against the murine cytomegalovirus (MCMV) m144 heavy chain
Comments
Animals were immunized with a gel slice of the m144 heavy chain and boosted two times.
Spleen cells were fused with HL-1 Friendly myeloma cells (a derivative of P3X63Ag8.653 that is deficient in both hypoxanthine phosphoribosyl transferase [HPRT] and adenine phosphoribosyl transferase [APRT]).
The antibody recognizes the murine cytomegalovirus (MCMV) m144 heavy chain.
The antibody can be used for ELISA, immunoprecipitation and Western blot applications.
Complete Growth Medium
HL-1 medium supplemented with 4 mM L-glutamine, 1 mM sodium pyruvate and 1% fetal bovine serum. HL-1 medium can be obtained from from Lonza (catalog number 77201).
Subculturing
Cultures can be maintained by addition of fresh Medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 x 105 viable cells/mL.  Maintain cultures at a cell concentration between 1 x 105 and 1 x 106 cells/mL.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
HL-1 Complete Serum-free medium described above supplemented with 10% (v/v) fetal bovine serum and 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype
IgG1
Name of Depositor
PJ Bjorkman
Deposited As
mouse (B cell); mouse (myeloma)
References

Chapman TL, Bjorkman PJ. Characterization of a murine cytomegalovirus class I major histocompatibility complex (MHC) homolog: comparison to MHC molecules and to the human cytomegalovirus MHC homolog. J. Virol. 72: 460-466, 1998. PubMed: 9420246

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

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