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16-10A1
16-10A1
規格:
價格:詢價
編號:B163589
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
16-10A1
商品貨號
B163589
Organism
hamster (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with and blocks the costimulatory activity of CD80.
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against murine CD80 (B7-1)
Cellular Products
immunoglobulin; monoclonal antibody; against murine CD80 (B7-1)
Comments
Animals were immunized with CD80 transfected CHO cells.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with and blocks the costimulatory activity of CD80.
It does not affect the activity of CD86 (B7-2).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Isotype
IgG
Name of Depositor
H Reiser, BW Janicki
Deposited As
hamster (B cell); mouse (myeloma)
References

Razi-Wolf Z, et al. Expression and function of the costimulatory molecule B7 on murine Langerhans cells: evidence for an alternative CTLA-4 ligand. Eur. J. Immunol. 24: 805-811, 1994. PubMed: 7512030

Razi-Wolf Z, et al. Expression and function of the murine B7 antigen, the major costimulatory molecule expressed by peritoneal exudate cells. Proc. Natl. Acad. Sci. USA 89: 4210-4214, 1992. PubMed: 1373896

Razi-Wolf Z, et al. Evidence for an additional ligand, distinct from B7, for the CTLA-4 receptor. Proc. Natl. Acad. Sci. USA 90: 11182-11186, 1993. PubMed: 7504299

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