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當前位置: 首頁 > ATCC代理 > 162-46.2 [Trop-2] B163593
162-46.2 [Trop-2]
162-46.2 [Trop-2]
規格:
價格:詢價
編號:B163593
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
162-46.2 [Trop-2]
商品貨號
B163593
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Choriocarcinoma
Derivation
Animals were immunized with BeWo cells (ATCC CCL-98).
Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against the trop-2 antigen of human trophoblasts cells and choriocarcinomas
Cellular Products
immunoglobulin; monoclonal antibody; against the trop-2 antigen of human trophoblasts cells and choriocarcinomas
Tumorigenic
Yes
Effects
Yes, in BALB/c mice
Comments
The antibody defines an antigen that is expressed on trophoblasts and choriocarcinoma cells but not on blood cells or other tumor cells.

Tested and found negative for ectromelia virus (mousepox).

Complete Growth Medium
Culture Medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain
1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium
pyruvate, 90%; fetal bovine serum, 10%.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage Temperature: Liquid nitrogen vapor phase
Isotype
IgG1 (Igh-4a allotype)
Name of Depositor
AM Stall
Deposited As
mouse (B cell); mouse (myeloma)
References

Lipinski M, et al. Human trophoblast cell-surface antigens defined by monoclonal antibodies. Proc. Natl. Acad. Sci. USA 78: 5147-5150, 1981. PubMed: 7029529

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