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17D
17D
規(guī)格:
價格:詢價
編號:B163604
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
17D
商品貨號
B163604
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody is specific for sheep CD4 and precipitates a 56000 dalton molecule from sheep T cell lysates.
Storage Conditions
liquid nitrogen vapor phase
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against ovine (sheep) CD4
Cellular Products
immunoglobulin; monoclonal antibody; against ovine (sheep) CD4
Comments
Animals were immunized with phytohemagglutinin activated sheep T cells.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody is specific for sheep CD4 and precipitates a 56000 dalton molecule from sheep T cell lysates.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10(5) cells/ml.
Interval: Maintain cultures at a cell concentration between 1 X 10(5) and 1 X 10(6) cells/ml
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype
mouse IgG1
Name of Depositor
CR Mackay
Deposited As
mouse (B cell); mouse (myeloma)
References

Mackay CR, et al. Unusual expression of CD2 in sheep: implications for T cell interactions. Eur. J. Immunol. 18: 1681-1688, 1988. PubMed: 2462499

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