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歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
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1A3.1
1A3.1
規格:
價格:
編號:B163613
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
1A3.1
商品貨號
B163613
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with G. muris trophozoite surfaces.
By immunofluorescence the antibody shows smooth surface fluorescence, staining of the flagella and aggregation of the trophozoites.
Derivation
Spleen cells were fused with P3/NSI/1-Ag4-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against Giardia muris trophozoites
Cellular Products
immunoglobulin; monoclonal antibody; against Giardia muris trophozoites
Comments
Animals were immunized with G. muris trophozoites.
Spleen cells were fused with P3/NSI/1-Ag4-1 myeloma cells.
The antibody reacts with G. muris trophozoite surfaces.
By immunofluorescence the antibody shows smooth surface fluorescence, staining of the flagella and aggregation of the trophozoites.
Complete Growth Medium
Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 80%; NCTC 109 medium, 10%; (Add 1 mM oxaloacetic acid, 0.45 mM sodium pyruvate, 4 mM L-glutamine, 0.1 mM hypoxanthine, 0.01 mM thymidine and 10 units/ml bovine insulin to this mixture); horse serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG3; kappa light chain
Name of Depositor
MF Heyworth
Deposited As
mouse (B cell); mouse (myeloma)
References

Heyworth MF, et al. Generation and characterization of monoclonal antibodies against Giardia muris trophozoites. Immunology 68: 341-345, 1989. PubMed: 2592009

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