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2254-62.2

規格:

價格:詢價

編號:B163643

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

2254-62.2

商品貨號

B163643

Organism

hamster

Tissue

kidney

培養基

Product Format

frozen

Morphology

fibroblast

Culture Properties

adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications

This line was derived from BHK-21(c-13) (see ATCC CCL-10).

This line has been used as a host for transformation with expression vectors containing selectable and amplifiable marker DNAs (e.

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Derivation

This line was derived from BHK-21(c-13) (see ATCC CCL-10).

Comments

This line was derived from BHK-21(c-13) (see ATCC CCL-10).

This line has been used as a host for transformation with expression vectors containing selectable and amplifiable marker DNAs (e.g., Factor VIII).

Complete Growth Medium

Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 100 nM methotrexate, 93%; dialyzed fetal bovine serum, 7%

Subculturing

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended

Medium Renewal: Every 2 to 3 days

Remove medium, rinse with fresh 0.25% trypsin, remove trypsin and incubate the flask at 37C until the cells detach (about 5 to 10 minutes). Add fresh medium, aspirate and dispense into new flasks.

Culture Conditions

Temperature: 37.0°C

Name of Depositor

Genentech, Inc.

Deposited As

hamster

U.S. Patent Number

4,965,199

References

Capon DJ, et al. Preparation of functional human factor VIII in mammalian cells using methotrexate based selection. US Patent 4,965,199 dated Oct 23 1990

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