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26ic
26ic
規(guī)格:
價格:詢價
編號:B163657
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
26ic
商品貨號
B163657
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with a nonfunctional domain of human CD14.
The binding of 26ic does not inhibit CD14 mediated activities, and is useful for detecting CD14 expression by immunofluorescence and or immunocytochemical methods.
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human CD14
Cellular Products
immunoglobulin; monoclonal antibody; against human CD14
Comments
Animals were immunized with cultured human peripheral blood monocytes.
Spleen cells were fused with NS-1 myeloma cells.
The antibody reacts with a nonfunctional domain of human CD14.
The binding of 26ic does not inhibit CD14 mediated activities, and is useful for detecting CD14 expression by immunofluorescence and or immunocytochemical methods.
Complete Growth Medium
The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
  • heat-inactivated gamma globulin free horse serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere

Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG2b; kappa light chain
Name of Depositor
RF Todd
Deposited As
mouse (B cell); mouse (myeloma)
References

Todd RF, et al. Structural analysis of differentiation antigens Mo1 and Mo2 on human monocytes. Hybridoma 1: 329-337, 1982. PubMed: 6208133

Wright SD, et al. CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein. Science 249: 1431-1433, 1990. PubMed: 1698311

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