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312-13E8
312-13E8
規(guī)格:
價(jià)格:詢價(jià)
編號:B163698
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
312-13E8
商品貨號
B163698
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Animals were immunized with a synthetic peptide with the sequence LMRACWQWNPSDRPSF corresponding to residues 471-486 of the abl oncogene (formerly 590 to 605 of the v-abl oncogene).
Images
Derivation
Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against a synthetic abl oncogene peptide
Cellular Products
immunoglobulin; monoclonal antibody; against a synthetic abl oncogene peptide
Comments
Animals were immunized with a synthetic peptide with the sequence LMRACWQWNPSDRPSF corresponding to residues 471-486 of the abl oncogene (formerly 590 to 605 of the v-abl oncogene).
Spleen cells were fused with Sp2/0 myeloma cells.
In ELISA testing, the antibody recognizes the fes, mos, erb B, src, fgr, yes, mil, raf, fms, and insulin receptor sequences in addition to the abl sequence. In immunoblot testing, a protein of approximately 80 kd is recognized in MOLT-4 cells.
The IgG1 reactivity of the antibody with the abl peptide (471 to 486) was confirmed by ELISA. IgG1 was strongly positive while IgG2b was negative.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype
IgG1 and IgG2b
Antigenic Determinant
abl oncogene peptide, synthetic
Name of Depositor
National Cancer Institute
Deposited As
mouse (B cell); mouse (myeloma)
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