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32B11

規格:

價格:詢價

編號:B163703

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

32B11

商品貨號

B163703

Organism

Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)

Cell Type

hybridoma: B lymphocyte

培養基

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications

The antibody reacts with glomalin on the surface of hyphae and spores and also on glomalin sloughed from hyphae.

Glomalin is a unique fungal protein secreted by the root-dwelling Glomales fungi through hairlike filaments called hyphae.

The antibody is used for studies on soil structure; it is useful in ELISA assays to detect glomalin on the surface of hyphae and glomalin in root cortical cells.

Storage Conditions

liquid nitrogen vapor phase

Derivation

Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.

Glomalin is a unique fungal protein secreted by the root-dwelling Glomales fungi through hairlike filaments called hyphae. It is found worldwide and it may the primary glue that holds soil together.

Genes Expressed

immunoglobulin; monoclonal antibody; against glomalin (a glycoprotein in soil)

Cellular Products

immunoglobulin; monoclonal antibody; against glomalin (a glycoprotein in soil)

Comments

Animals were immunized with freshly collected Glomus intraradices FL208 spores.

Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.

The antibody reacts with glomalin on the surface of hyphae and spores and also on glomalin sloughed from hyphae.

The antibody is slightly cross-reactive with Leptospharia korrea.

Glomalin is a unique fungal protein secreted by the root-dwelling Glomales fungi through hairlike filaments called hyphae. It is found worldwide and it may the primary glue that holds soil together.

The antibody is used for studies on soil structure; it is useful in ELISA assays to detect glomalin on the surface of hyphae and glomalin in root cortical cells.

Complete Growth Medium

RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.1 mM hypoxanthine and 0.016 mM thymidine, 90%; fetal bovine serum, 10%

Subculturing

Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml. Attached cells may be subcultured by scraping. Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Cryopreservation

Freeze medium: culture medium 92.5%; DMSO, 7.5%

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37.0°C

Isotype

IgM; kappa light chain

Name of Depositor

SF Wright

Deposited As

mouse (B cell); mouse (myeloma)

References

Wright SF, et al. Time-course study and partial characterization of a protein on hyphae of arbuscular mycorrhizal fungi during active colonization of roots. Plant Soil 181: 193-203, 1996.

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