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33D1
33D1
規格:
價格:詢價
編號:B163707
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
33D1
商品貨號
B163707
Organism
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with P3X63Ag8-U1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse dendritic cells (cytotoxic)
Cellular Products
immunoglobulin; monoclonal antibody; against mouse dendritic cells (cytotoxic)
Comments
Animals were immunized with mouse spleen and lymph node dendritic cells.
Spleen cells were fused with P3X63Ag8-U1 myeloma cells.
The antigen recognized by the 33D1 antibody appears to be present only on dendritic cells (10000 to 15000 sites/cell).
The antigen does not appear to be immune response associated antigen or Ia antigen.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG2b
Name of Depositor
RM Steinman
Deposited As
rat (B cell); mouse (myeloma)
References

Nussenzweig MC, et al. A monoclonal antibody specific for mouse dendritic cells. Proc. Natl. Acad. Sci. USA 79: 161-165, 1982. PubMed: 6948298

Steinman RM, et al. Dendritic cells are the principal stimulators of the primary mixed leukocyte reaction in mice. J. Exp. Med. 157: 613-627, 1983. PubMed: 6185614

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