黄色小说视频-日本少妇高潮抽搐-国内自拍av-天堂中文资源在线-蜜桃视频在线入口www-91久久综合-亚洲视频在线一区-日日夜夜拍-国产精品资源在线观看-欧美激情国产精品免费-人妻少妇久久中文字幕-成人免费无码大片a毛片-香蕉污视频在线观看-日本电影成人-国内精品久久99人妻无码

歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > 413-15D12 B163746
413-15D12
413-15D12
規(guī)格:
價格:詢價
編號:B163746
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
413-15D12
商品貨號
B163746
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Animals were immunized with a synthetic polypeptide with the sequence LSPEEEEKRRIRRERNKMAAAKC corresponding to peptides 132-154 of the c-fos oncogene.
The antibody reacts with the synthetic c-fos oncogene peptide.
In ELISA testing, it cross-reacts weakly with the r-fos peptide.
In immunoblot testing a protein of approximately 70 kd is detected in duck cells infected with v-fos.
Derivation
Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against a synthetic fos oncogene peptide
Cellular Products
immunoglobulin; monoclonal antibody; against a synthetic fos oncogene peptide
Comments
Animals were immunized with a synthetic polypeptide with the sequence LSPEEEEKRRIRRERNKMAAAKC corresponding to peptides 132-154 of the c-fos oncogene.
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody reacts with the synthetic c-fos oncogene peptide. In ELISA testing, it cross-reacts weakly with the r-fos peptide.
In immunoblot testing a protein of approximately 70 kd is detected in duck cells infected with v-fos. A protein of approximately 35 kd is also detected in this cell line as well as several additional cell lines, such as ANN-1, MSTF, A431, and SSV3T3.
The IgG1 reactivity of the antibody with the c-fos peptide (132-154) was confirmed by ELISA.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml.
Maintain cell density between 2 X 10 exp5 and 2 X 10 exp6 viable cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype
IgG1 and IgG2b
Name of Depositor
National Cancer Institute
Deposited As
mouse (B cell); mouse (myeloma)
  • 王經(jīng)理
    手機號：17280875617
    QQ：1438578920
  • 胡經(jīng)理
    手機號：13345964880
    QQ：2438244627
  • 周經(jīng)理
    手機號：17757487661
    QQ：1296385441
  • 于經(jīng)理
    手機號：18067160830
    QQ：2088210172
  • 沈經(jīng)理
    手機號：19548299266
    QQ：2662369050
  • 李經(jīng)理
    手機號：13626845108
    QQ：972239479
  • 曹經(jīng)理
    手機號：18662422060
    QQ：3671869992