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44aacb
44aacb
規(guī)格:
價(jià)格:詢(xún)價(jià)
編號(hào):B163749
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng)
44aacb
商品貨號(hào)
B163749
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Leukemia
Applications
The antibody reacts with human CD11b, and blocks a variety of CD11b/CD18 mediated activities including neutrophil - substrate adherence and C3bi rosette formation on neutrophils, monocytes, macrophages and some NK cells.
The antibody cross-reacts with CD11b from non-human primates.
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human CD11b
Cellular Products
immunoglobulin; monoclonal antibody; against human CD11b
Comments
Animals were immunized with human acute monocytic leukemia cells (FAM M6).
Spleen cells were fused with NS-1 myeloma cells.
The antibody reacts with human CD11b, and blocks a variety of CD11b/CD18 mediated activities including neutrophil - substrate adherence and C3bi rosette formation on neutrophils, monocytes, macrophages and some NK cells.
The antibody cross-reacts with CD11b from non-human primates.
Complete Growth Medium
The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
  • heat-inactivated gamma globulin free horse serum to a final concentration of 10%
    • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere

Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG2a; kappa light chain
Name of Depositor
RF Todd
Deposited As
mouse (B cell); mouse (myeloma)
References

Arnaout MA, et al. Inhibition of phagocytosis of complement C3- or immunoglobulin G-coated particles and of C3bi binding by monoclonal antibodies to a monocyte- granulocyte membrane glycoprotein (Mol). J. Clin. Invest. 72: 171-179, 1983. PubMed: 6874946

Simon RH, et al. Neutrophil-induced injury of rat pulmonary alveolar epithelial cells. J. Clin. Invest. 78: 1375-1386, 1986. PubMed: 3771800

Ismail G, et al. Prevention of pulmonary injury in isolated perfused rat lungs by activated human neutrophils preincubated with anti-Mo1 monoclonal antibody. Blood 69: 1167-1174, 1987. PubMed: 3030466

Dana N, et al. Two functional domains in the phagocyte membrane glycoprotein Mo1 identified with monoclonal antibodies. J. Immunol. 137: 3259-3263, 1986. PubMed: 2430017

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