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454C11
454C11
規(guī)格:
價格:詢價
編號:B163750
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
454C11
商品貨號
B163750
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Cancer,Cancer
Applications
The antibody reacts with a 210000 dalton protein found in cancerous human breast tissue.
By immunofluorescence, the antibody reacts with epithelia of several normal and neoplastic tissues.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human breast cancer cells
Cellular Products
immunoglobulin; monoclonal antibody; against human breast cancer cells
Tumorigenic
Yes
Effects
Yes, forms ascites in BALB/c mice
Comments
Animals were immunized either with live cells or with membrane extracts from human breast cancer cell lines.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with a 210000 dalton protein found in cancerous human breast tissue.
By immunofluorescence, the antibody reacts with epithelia of several normal and neoplastic tissues.
Complete Growth Medium
Culture Medium: Dulbecco's modified Eagle's medium (see below), with HAT 80%;
fetal bovine serum, 20%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG2a
Name of Depositor
Cetus Corp.
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Ring DB, et al. Monoclonal anti-human breast cancer antibodies. US Patent 4,753,894 dated Jun 28 1988

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