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4B2
4B2
規格:
價格:詢價
編號:B163759
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
4B2
商品貨號
B163759
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications

The antibody reacts with all types of human leukocytes, but not with non-leukocytes.

The antibody binds to protein A.

Storage Conditions
liquid nitrogen vapor phase
Derivation
Animals were immunized with human T cells.

Spleen cells were fused with P3U1 myeloma cells.

Genes Expressed
immunoglobulin; monoclonal antibody; against the human T200 common leukocyte antigen (CD45)
Cellular Products
immunoglobulin; monoclonal antibody; against the human T200 common leukocyte antigen (CD45)
Tumorigenic
Yes, in irradiated pristane primed (BALB/c X DBA/2)F1 mice.
Comments

The antibody reacts with all types of human leukocytes, but not with non-leukocytes.

The antibody binds to protein A.

Tested and found negative for ectromelia virus (mousepox).

Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 10viable cells/mL. Maintain cultures at a cell concentration between 2 x 105 and 1 x 10cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC® Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Isotype
IgG2a
Name of Depositor
RM Steinman
Deposited As
mouse (B cell); mouse (myeloma)
References

. Leucocyte typing III: white cell differentiation antigens. Oxford: Oxford Univ. Press; 1987.

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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