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4E8C12
4E8C12
規格:
價格:
編號:B163769
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
4E8C12
商品貨號
B163769
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Animals were immunized with a rough strain of O157:H7 (Escherichia coli HA1, deficient in the O157 antigen).
The antibody is specific for E. coli O157:H7 and O26:H11 both of which are enterohemorrhagic.
It reacts with a protein antigen of 13000 daltons.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against an enterohemorrhagic strain of Escherichia coli (E. coli O157:H7 strain 932)
Cellular Products
immunoglobulin; monoclonal antibody; against an enterohemorrhagic strain of Escherichia coli (E. coli O157:H7 strain 932)
Tumorigenic
Yes
Effects
Yes, forms ascites in pristane primed BALB/c mice
Comments
Animals were immunized with a rough strain of O157:H7 (Escherichia coli HA1, deficient in the O157 antigen).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody is specific for E. coli O157:H7 and O26:H11 both of which are enterohemorrhagic.
It reacts with a protein antigen of 13000 daltons.
Complete Growth Medium

Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
culture medium, 90%; DMSO, 10%
Isotype
Secretes a mouse IgG2a monoclonal antibody specific for enteropathogenic
Escherichia coli serotypes O157:H7 and O26:H11.
Name of Depositor
Wisconsin Alumni Res. Fndn.
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Doyle MP, Padhye N. Monoclonal antibody to enterohemorrhagic Escherichia coli 0157:H7 and 026:H11. US Patent 5,168,063 dated Dec 1 1992

Doyle MP, Padhye N. Monoclonal antibody to enterohemorrhagic Escherichia coli 0157:H7 and 026:H11 and method for detection. US Patent 5,354,661 dated Oct 11 1994

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