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5/9 m alpha3-18
5/9 m alpha3-18
規格:
價格:詢價
編號:B163773
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
5/9 m alpha3-18
商品貨號
B163773
Organism
Cricetulus griseus, hamster, Chinese
Tissue
ovary
培養基
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Gender
female
Storage Conditions
liquid nitrogen vapor phase
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
This line was derived from the CHO DUKX cell line.
Clinical Data
female
Genes Expressed
human colony stimulating factor (M-CSF)
Cellular Products
human colony stimulating factor (M-CSF)
Comments
The cells are resistant to methotrexate.
Complete Growth Medium
Alpha minimum essential medium with 2 mM L-glutamine and 1 mM sodium pyruvate without ribonucleosides and deoxyribonucleosides supplemented with 200 nM MTX (Methotrexate) and 10% dialyzed fetal bovine serum (Sigma F0392)
Subculturing
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:10 to 1:20 is recommended
Medium Renewal: Two to three times weely
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Name of Depositor
Genetics Institute, Inc.
Deposited As
Cricetulus griseus
U.S. Patent Number
References

Clark SC, Wong GG. Hematopoietic growth factors. US Patent 4,868,119 dated Sep 19 1989

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