黄色小说视频-日本少妇高潮抽搐-国内自拍av-天堂中文资源在线-蜜桃视频在线入口www-91久久综合-亚洲视频在线一区-日日夜夜拍-国产精品资源在线观看-欧美激情国产精品免费-人妻少妇久久中文字幕-成人免费无码大片a毛片-香蕉污视频在线观看-日本电影成人-国内精品久久99人妻无码

歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > 548 B163782
548
548
規(guī)格:
價(jià)格:詢價(jià)
編號:B163782
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
548
商品貨號
B163782
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Leukemia
Applications
The antibody reacts with the p12 protein of most MuLV.
The antibody reacts with infected cells after acetone or methanol fixation and immunoprecipitates p12.
Storage Conditions
liquid nitrogen vapor phase
Derivation
Spleen cells from a mouse recovering from splenomegaly induced by Moloney murine leukemia virus (MoMuLV) and Spleen Focus Forming virus (SFFV) were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against p12 group antigen (GAG) protein of murine leukemia viruses (MuLV)
Cellular Products
immunoglobulin; monoclonal antibody; against p12 group antigen (GAG) protein of murine leukemia viruses (MuLV)
Comments
Spleen cells from a mouse recovering from splenomegaly induced by Moloney murine leukemia virus (MoMuLV) and Spleen Focus Forming virus (SFFV) were fused with NS-1 myeloma cells.
The antibody reacts with the p12 protein of most MuLV.
The antibody reacts with infected cells after acetone or methanol fixation and immunoprecipitates p12.
Complete Growth Medium
RPMI 1640 medium with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 105 cells/mL and maintain between 1 x 105 and 1 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype
IgG2b
Name of Depositor
B Chesebro
Deposited As
mouse (B cell); mouse (myeloma)
References

Chesebro B, et al. Characterization of monoclonal antibodies reactive with murine leukemia viruses: Use in analysis of strains of Friend MCF and Friend ecotropic murine leukemia virus. Virology 127: 134-148, 1983. PubMed: 6305011

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

  • 王經(jīng)理
    手機(jī)號:17280875617
    QQ:1438578920
  • 胡經(jīng)理
    手機(jī)號:13345964880
    QQ:2438244627
  • 周經(jīng)理
    手機(jī)號:17757487661
    QQ:1296385441
  • 于經(jīng)理
    手機(jī)號:18067160830
    QQ:2088210172
  • 沈經(jīng)理
    手機(jī)號:19548299266
    QQ:2662369050
  • 李經(jīng)理
    手機(jī)號:13626845108
    QQ:972239479
  • 曹經(jīng)理
    手機(jī)號:18662422060
    QQ:3671869992