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5H8

規(guī)格:

價格:詢價

編號:B163798

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

5H8

商品貨號

B163798

Organism

Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)

Cell Type

hybridoma: B lymphocyte

培養(yǎng)基

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

Carcinoma

Applications

The antibody reacts with periostin, a secreted protein.

Storage Conditions

liquid nitrogen vapor phase

Derivation

Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells.

Clinical Data

The levels of serum periostin may be an indicator of tumor invasion and progression in patients with thymoma and nonsmall cell lung carcinomas (NSCLC).

Genes Expressed

immunoglobulin; monoclonal antibody; against periostin

Cellular Products

immunoglobulin; monoclonal antibody; against periostin

Comments

The antibody reacts with periostin, a secreted protein. The levels of serum periostin may be an indicator of tumor invasion and progression in patients with thymoma and nonsmall cell lung carcinomas (NSCLC).

Animals were immunized with Fc-TCG1, which is similar to periostsin.

Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Note: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.

Cryopreservation

Freeze medium: A 1:1 mixture of culture medium and fetal bovine serum, 90%; DMSO, 10%

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37°C

Isotype

IgG1; kappa light chain

Name of Depositor

LB Chen

Deposited As

mouse (B cell); mouse (myeloma)

References

Sasaki H, et al. Serum level of the periostin, a homologue of an insect cell adhesion molecule, in thymoma patients. Cancer Lett. 172: 37-42, 2001. PubMed: 11595127

Sasaki H, et al. Serum level of the periostin, a homologue of an insect cell adhesion molecule, as a prognostic marker in nonsmall cell lung carcinomas. Cancer 92: 843-848, 2001. PubMed: 11550156

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