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歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
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6-23 (Clone 6)
6-23 (Clone 6)
規格:
價格:詢價
編號:B163800
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
6-23 (Clone 6)
商品貨號
B163800
Organism
Rattus norvegicus, rat
Tissue
thyroid
培養基
Cell Type
C cell
Product Format
frozen
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
medullary thyroid carcinoma
Storage Conditions
liquid nitrogen vapor phase
Genes Expressed
neurotensin; calcitonin
Comments
Secretion of neurotensin and calcitonin is stimulated by calcium, KCl, glucagon and catecholamines.
Complete Growth Medium
Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 85%; horse serum, 15%
Subculturing
Remove medium, add fresh 0.25% trypsin, 0.02% EDTA solution for 1 minute, remove trypsin EDTA, add 0.3 mL of 0.25% trypsin and let the culture sit at 37°C for 6 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 10 to 12 days.
Subcultivation Ratio: A subcultivation ratio of 1:10 to 1:20 is recommended
Medium Renewal: 3 times per week
Cryopreservation
Freeze medium: complete growth medium, 95%; DMSO, 5%
Name of Depositor
R Gagel, FN Zeytinoglu
Deposited As
Rattus sp.
References

Zeytinoglu FN, et al. Establishment of a calcitonin-producing rat medullary thyroid carcinoma cell line. Morphological studies of the tumor and cells in culture. Endocrinology 107: 509-515, 1980. PubMed: 6248326

Zeytinoglu FN, et al. Characterization of neurotensin production by a line of rat medullary thyroid carcinoma cells. Proc. Natl. Acad. Sci. USA 77: 3741-3745, 1980. PubMed: 6932046

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