黄色小说视频-日本少妇高潮抽搐-国内自拍av-天堂中文资源在线-蜜桃视频在线入口www-91久久综合-亚洲视频在线一区-日日夜夜拍-国产精品资源在线观看-欧美激情国产精品免费-人妻少妇久久中文字幕-成人免费无码大片a毛片-香蕉污视频在线观看-日本电影成人-国内精品久久99人妻无码

歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購(gòu)物車 1 種商品 - 共0元
當(dāng)前位置: 首頁(yè) > ATCC代理 > 68-1-2 B163803
68-1-2
68-1-2
規(guī)格:
價(jià)格:詢價(jià)
編號(hào):B163803
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
68-1-2
商品貨號(hào)
B163803
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody is specific for the alpha subunit and recognizes a determinant present only on the denatured enzyme.
Storage Conditions
liquid nitrogen vapor phase
Derivation
Mice were immunized with E. coli DNA polymerase III' containing the alpha, epsilon, theta and tau subunits. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody is specific for the alpha subunit and recognizes a determinant present only on the denatured enzyme. The site recognized by the 68-1-2 antibody is distinct from that recognized by 123-10 (ATCC CRL-1707) but is adjacent to, or interacts with, the 123-10 site.
Comments
Mice were immunized with E. coli DNA polymerase III' containing the alpha, epsilon, theta and tau subunits. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody is specific for the alpha subunit and recognizes a determinant present only on the denatured enzyme. The site recognized by the 68-1-2 antibody is distinct from that recognized by 123-10 (ATCC CRL-1707) but is adjacent to, or interacts with, the 123-10 site.
Complete Growth Medium
Iscove's modified Dulbecco's medium, 85%; fetal bovine serum, 15%
Subculturing
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype
IgM
Name of Depositor
M Franden, CS McHenry
Deposited As
mouse (B cell); mouse (myeloma)
References

Wu YH, et al. Monoclonal antibodies specific for the alpha subunit of the Escherichia coli DNA polymerase III holoenzyme. J. Biol. Chem. 259: 12117-12122, 1984. PubMed: 6090455

The determinant is present only on the denatured enzyme

  • 王經(jīng)理
    手機(jī)號(hào)：17280875617
    QQ：1438578920
  • 胡經(jīng)理
    手機(jī)號(hào)：13345964880
    QQ：2438244627
  • 周經(jīng)理
    手機(jī)號(hào)：17757487661
    QQ：1296385441
  • 于經(jīng)理
    手機(jī)號(hào)：18067160830
    QQ：2088210172
  • 沈經(jīng)理
    手機(jī)號(hào)：19548299266
    QQ：2662369050
  • 李經(jīng)理
    手機(jī)號(hào)：13626845108
    QQ：972239479
  • 曹經(jīng)理
    手機(jī)號(hào)：18662422060
    QQ：3671869992