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7C2C5C12
7C2C5C12
規(guī)格:
價格:詢價
編號:B163824
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
7C2C5C12
商品貨號
B163824
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Tissue
spleen
培養(yǎng)基
Cell Type
hybridoma: B lymphocyte
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain
BALB/c
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against Trichinella spiralis
Cellular Products
immunoglobulin; monoclonal antibody; against Trichinella spiralis
Tumorigenic
Yes
Effects
Yes, forms ascites in BALB/c mice
Comments
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Complete Growth Medium
Culture Medium: Iscove's modified Dulbecco's medium, 80%; fetal bovine serum,
20%.
Subculturing
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
Culture Conditions
Temperature: 37.0°C
Isotype
IgM
Name of Depositor
USDA
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Gamble HR, Graham CE. Monoclonal antibody-purified antigen for the immunodiagnosis of trichinosis. Am. J. Vet. Res. 45: 67-74, 1984. PubMed: 6703457

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