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7C8

規格:

價格:詢價

編號:B163825

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

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產品名稱

7C8

商品貨號

B163825

Organism

Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)

Cell Type

hybridoma: B lymphocyte

培養基

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain:? BALB/c (B cell); BALB/c (myeloma)

Applications

The antibody can be used to detect and purify proteins produced in an expression system using a a vector containing the sequence coding for the polypeptide.

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Derivation

Spleen cells were fused with NS-1 myeloma cells.

The antibody can be used to detect and purify proteins produced in an expression system using a a vector containing the sequence coding for the polypeptide.

Genes Expressed

immunoglobulin; monoclonal antibody; against a synthetic polypeptide (NH2-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-Gly-Pro-Lys-Lys-Gly-COOH)

Cellular Products

immunoglobulin; monoclonal antibody; against a synthetic polypeptide (NH2-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-Gly-Pro-Lys-Lys-Gly-COOH)

Tumorigenic

Yes

Effects

Yes, forms ascites in pristane primed BALB/c mice

Comments

Animals were immunized with the above synthetic polypeptide.

Spleen cells were fused with NS-1 myeloma cells.

The antibody can be used to detect and purify proteins produced in an expression system using a a vector containing the sequence coding for the polypeptide.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.

Subculturing

Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 5 X 10 exp5 viable cells/ml.

Medium Renewal: Every 2 to 3 days

Culture Conditions

Temperature: 37.0°C

Isotype

IgM

Name of Depositor

Immunex Corporation

Deposited As

mouse (B cell); mouse (myeloma)

U.S. Patent Number

4,703,004

References

Hopp TP, et al. Synthesis of protein with an identification peptide. US Patent 4,703,004 dated Oct 27 1987

Hopp TP, et al. Synthesis of protein with an identification peptide, and hybrid polypeptide incorporating same. US Patent 4,782,137 dated Nov 1 1988

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