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當前位置: 首頁 > ATCC代理 > 7E11C5 [7E11-C5] B163829
7E11C5 [7E11-C5]
7E11C5 [7E11-C5]
規格:
價格:詢價
編號:B163829
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
7E11C5 [7E11-C5]
商品貨號
B163829
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Cancer
Strain

Strain:? BALB/c (B cell); BALB/c (myeloma)

Applications
The antibody reacts selectively with cells from both normal and neoplastic prostatic epithelium.
Storage Conditions
liquid nitrogen vapor temperature
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Animals were immunized with live LNCaP cells and purified plasma membranes from LNCaP cells.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Clinical Data
The antigen recognized by 7E11C5 appears to be present in sera from patients with prostate cancer.
Genes Expressed
immunoglobulin; monoclonal antibody; against human prostate cancer antigen
Cellular Products
immunoglobulin; monoclonal antibody; against human prostate cancer antigen
Tumorigenic
Yes
Effects
Yes, forms ascites in pristane primed BALB/c mice
Comments
Animals were immunized with live LNCaP cells and purified plasma membranes from LNCaP cells.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts selectively with cells from both normal and neoplastic prostatic epithelium.
By immunofluorescence staining, the reaction was much stronger with neoplastic than normal epithelium.
The antigen recognized by 7E11C5 appears to be present in sera from patients with prostate cancer.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium, 90%; DMSO, 10%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions
Temperature: 37.0°C
Isotype
IgG1
Name of Depositor
Cytogen Corporation
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Horoszewicz JS. Hybridoma and monoclonal antibody 9H10-A4 specific to an antigen of LNCaP cells. US Patent 5,578,484 dated Nov 26 1996

Horoszewicz JS. Monoclonal antibodies to a new antigenic marker in epithelial prostatic cells and serum of prostatic cancer patients. US Patent 5,162,504 dated Nov 10 1992

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