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80 V 5B4
80 V 5B4
規(guī)格:
價(jià)格:詢(xún)價(jià)
編號(hào):B163833
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng)
80 V 5B4
商品貨號(hào)
B163833
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Tested and found negative for ectromelia virus (mousepox).
Derivation
Animals were immunized with DEAE purified 79 X1 C2 hybridoma product conjugated to keyhole limpet hemocyanin.
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against an idiotypic determinant (idiotope, idiotype) on the P3X63Ag8 myeloma protein
Cellular Products
immunoglobulin; monoclonal antibody; against an idiotypic determinant (idiotope, idiotype) on the P3X63Ag8 myeloma protein
Comments
Animals were immunized with DEAE purified 79 X1 C2 hybridoma product conjugated to keyhole limpet hemocyanin.
Spleen cells were fused with NS-1 myeloma cells.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Isotype
IgG2b; kappa light chain
Name of Depositor
J Gheuens
Deposited As
mouse (B cell); mouse (myeloma)
References

Gheuens J, McFarlin DE. Use of monoclonal anti-idiotypic antibody to P3/X63/Ag8-myeloma protein for analysis and purification of B-lymphocyte hybridoma products. Eur. J. Immunol. 12: 701-703, 1982. PubMed: 6982818

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