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8A2N
8A2N
規(guī)格:
價(jià)格:詢價(jià)
編號(hào):B163842
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
8A2N
商品貨號(hào)
B163842
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Animals were immunized with type O, Rh- human erythrocytes and purified glycophorin A.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against glycophorin A type N
Cellular Products
immunoglobulin; monoclonal antibody; against glycophorin A type N
Tumorigenic
Yes
Effects
Yes, forms ascites in immunosuppressed BALB/c mice
Comments
Animals were immunized with type O, Rh- human erythrocytes and purified glycophorin A.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody does not react with type M glycophorin A.
Complete Growth Medium
Culture Medium: Dulbecco's modified Eagle's medium, 90%; fetal bovine serum,
10%.
Subculturing
Medium Renewal: 2 to 3 times per week
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 70%; heat-inactivated fetal bovine serum, 20%; DMSO, 10%
Isotype
IgG1; kappa light chain
Name of Depositor
Lawrence Livermore National Lab
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Bigbee WL, et al. Monoclonal antibodies specific for the M-and N-forms of human glycophorin A. Mol. Immunol. 20: 1353-1362, 1983. PubMed: 6197636

Bigbee WL, et al. Binding specificities of eight monoclonal antibodies to human glycophorin A. Studies with McM, and MkEn(UK) variant human erythrocytes and M- and MNv-type chimpanzee erythrocytes. J. Immunol. 133: 3149-3155, 1984. PubMed: 6491283

Bigbee WL, et al. Cell lines for the production of monoclonal antibodies to human glycophorin A. US Patent 4,767,710 dated Aug 30 1988

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